Cellular Uptake and Localization of Polymyxins in Renal Tubular Cells Using Rationally Designed Fluorescent Probes
نویسندگان
چکیده
منابع مشابه
Cellular Uptake and Localization of Polymyxins in Renal Tubular Cells Using Rationally Designed Fluorescent Probes.
Polymyxins are cyclic lipopeptide antibiotics that serve as a last line of defense against Gram-negative bacterial superbugs. However, the extensive accumulation of polymyxins in renal tubular cells can lead to nephrotoxicity, which is the major dose-limiting factor in clinical use. In order to gain further insights into the mechanism of polymyxin-induced nephrotoxicity, we have rationally desi...
متن کاملRationally-designed fluorescent lysine riboswitch probes.
Two fluorescent lysine amide analogs, in which the carboxyl end of lysine was covalently attached to dansyl or NBD groups through an ethylene glycol-based linker, were rationally designed and synthesized. Both probes showed high binding affinity to the lysine riboswitch in vitro and their fluorescence intensities decreased by riboswitch binding.
متن کاملVisualizing chemical structure-subcellular localization relationships using fluorescent small molecules as probes of cellular transport
BACKGROUND To study the chemical determinants of small molecule transport inside cells, it is crucial to visualize relationships between the chemical structure of small molecules and their associated subcellular distribution patterns. For this purpose, we experimented with cells incubated with a synthetic combinatorial library of fluorescent, membrane-permeant small molecule chemical agents. Wi...
متن کاملFluorescence detection of natural RNA using rationally designed “clickable” oligonucleotide probes
Interdisciplinary Nanoscience Center (iNAN and Genetics, Aarhus University, Gustav W E-mail: [email protected]; Tel: +45 28992086 Nucleic Acid Center, Department of Physics Southern Denmark, DK-5230 Odense M, Denma † Electronic supplementary information purication and characterization of the limit of target detection calculations, an targeting complementary and singly-mis 10.1039/c4ra07165d Cite thi...
متن کاملFluorescent probes of the apoptolidins and their utility in cellular localization studies.
Apoptolidin A has been described among the top 0.1% most-cell-selective cytotoxic agents to be evaluated in the NCI 60 cell line panel. The molecular structure of apoptolidin A consists of a 20-membered macrolide with mono- and disaccharide moieties. In contrast to apoptolidin A, the aglycone (apoptolidinone) shows no cytotoxicity (>10 μM) when evaluated against several tumor cell lines. Apopto...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Antimicrobial Agents and Chemotherapy
سال: 2015
ISSN: 0066-4804,1098-6596
DOI: 10.1128/aac.01216-15